http://currentprotocolsblog.wordpress.com/2008/05/05/question-regarding-transient-expression-of-proteins-using-cos-cells/ Fri, 08 May 2009 10:41:42 +0000 http://wordpress.com/ hourly 1 http://currentprotocolsblog.wordpress.com/2008/05/05/question-regarding-transient-expression-of-proteins-using-cos-cells/#comment-3 rburley Tue, 06 May 2008 15:57:17 +0000 http://currentprotocolsblog.wordpress.com/?p=29#comment-3 Some investigators do in fact add these washing steps to the procedure and find that they have some impact on the efficiency of transfection. The authors do not use the washes and did not include them in the protocol because they add too many steps to the procedure and their value was found to be marginal. Some investigators do in fact add these washing steps to the procedure and find that they have some impact on the efficiency of transfection. The authors do not use the washes and did not include them in the protocol because they add too many steps to the procedure and their value was found to be marginal.

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